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Frederick Settle Barff, the renowned English chemist born in Hackney, Greater London in 1823, made significant contributions to the field of science. One of his notable achievements was the discovery and study of activated granulocytes using scanning electron microscopy (SEM). Activated granulocytes are a type of white blood cell that play a crucial role in our immune system's defense against infections and diseases. Barff's pioneering work allowed scientists to observe these cells at an unprecedented level of detail. Through SEM imaging, Barff revealed the intricate structures and functions of activated granulocytes. These images showcased their unique features such as irregularly shaped nuclei and numerous cytoplasmic granules. The high-resolution SEM images captured by Barff provided valuable insights into how activated granulocytes interact with pathogens. They showed these cells engulfing bacteria or releasing toxic substances to neutralize threats within our bodies. Barff's research on activated granulocytes paved the way for further advancements in understanding immune responses and developing new treatments for various illnesses. His dedication to scientific exploration has left an indelible mark on the field of immunology. Today, thanks to Frederick Settle Barff's groundbreaking work, scientists continue to delve deeper into studying activated granulocytes using advanced imaging techniques like SEM. This ongoing research holds immense potential for improving our understanding of immune system disorders and finding innovative ways to combat them.