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False colour SEM of Sephadex beads
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False colour SEM of Sephadex beads
False colour scanning electron micrograph of Sephadex beads. These beads are used in the separation of molecules according to size (molecular weight). They are made of an inert polymer, in this case dextran, and each bead contains pores of a specific size. A glass column is packed with beads in water and the mixture of proteins, or protein and salt, to be separated is poured in. Small molecules can penetrate the pores in the matrix and their progress through the column is impeded, while larger molecules proceed downwards at the rate of flow of the solvent. This technique is known as column chromotography or gel filtration. Magnification: x200 at 6x4.5cm size
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Media ID 6439237
© DR JEREMY BURGESS/SCIENCE PHOTO LIBRARY
Apparatus Column Chromatography Proteomics
EDITORS COMMENTS
This print showcases a false color scanning electron micrograph of Sephadex beads, revealing the intricate details of these remarkable molecular separation tools. Used extensively in the field of proteomics, Sephadex beads are composed of an inert polymer called dextran and possess pores with specific sizes. The glass column depicted in the image is densely packed with these beads immersed in water. The technique employed here is known as column chromatography or gel filtration, which enables scientists to separate molecules based on their size or molecular weight. When a mixture containing proteins or protein and salt is poured into the column, smaller molecules can infiltrate the pores within the matrix but face resistance that impedes their progress through the column. In contrast, larger molecules flow downwards at a rate determined by the solvent. With its magnification set at x200 for this 6x4.5cm print, this photograph offers us an up-close view of Sephadex beads and their crucial role in scientific research. By harnessing their unique properties, researchers can effectively fractionate proteins and gain valuable insights into various biological processes. This stunning visual representation from Science Photo Library not only highlights cutting-edge technology and apparatus used in chemistry labs but also underscores how industry professionals utilize gel filtration techniques to advance our understanding of complex biomolecular interactions without mentioning any commercial applications associated with it.
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