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Directed differentiation of multipotential human neural progenitor cells
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Directed differentiation of multipotential human neural progenitor cells
Human neural progenitor cells were isolated under selective culture conditions from the developing human brain and directed through lineage differentiation to GFAP + (glial fibrillary acid protein) astrocytes. Following 3 weeks in astrocyte selective medium, cells were fixed and stained with antibodies to intermediate filament proteins that characterize the cells as either astrocytes (GFAP in orange) or neural progenitor cells (nestin in green). Nuclei are stained with DAPI in blue. Microscopic examination over the course of differentiation showed loss of progenitor cells (nestin down regulation) as cell population increasingly became astrocytes (GFAP up regulation). Photomicrograph was taken at 200x magnification
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This print showcases the directed differentiation of multipotential human neural progenitor cells. These remarkable cells were isolated from the developing human brain and guided through lineage differentiation to become GFAP+ astrocytes, as depicted by the vibrant orange staining of glial fibrillary acid protein (GFAP). The green staining with nestin represents the neural progenitor cells that gradually decrease in number during this process. The image was captured at a magnification of 200x using microscopy techniques. It provides a close-up view of these specialized cells, highlighting their intricate cytoskeleton and nuclei stained blue with DAPI. Through microscopic examination over several weeks, it becomes evident how the population shifts from predominantly neural progenitor cells to an abundance of mature astrocytes. This photomicrograph holds great significance in medical research and neuroscience, shedding light on neurogenesis, nervous system development, and cell differentiation. It offers valuable insights into the growth and behavior of these crucial components within our brains. With its rich colors and detailed composition, this image serves as a powerful visual representation of scientific progress in understanding stem cell biology and its potential applications in healthcare. Its inclusion in National Institutes of Health's collection further emphasizes its importance for advancing biomedical knowledge without any commercial intent.
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